Ion chromatography or ion-exchange chromatography is a procedure that Permits the separation of ions and polar molecules on the basis of the charge properties of these molecules. It may be used for many types of charged molecules such as big Proteins, little nucleotides and amino acids. The solution to be injected is normally known as a sample and the independently separated components are identified as analytes. It is often utilized in protein purification, water analysis and for quality control purposes. Ion exchange chromatography keeps analyte molecules using coulombic ionic interactions. The stationary phase surface exhibits ionic functional groups that interact with analyte ions of opposite charge. This category of chromatography can be further subdivided into cation exchange chromatography and anion exchange chromatography. The ionic compound comprising the cationic species and the anionic species may be retained by the stationary phase.
Cation exchange ion chromatography keeps positively charged cations Because the stationary phase exhibits a negatively charged functional category. Anion exchange chromatography keeps anions displaying a positively charged functional class. Note that the ion power of cations or anions in the mobile phase may be adjusted to alter the balance position and, hence, the retention period. An ion chromatogram can be used to reveal that the chromatogram obtained with an ion exchange column. A Normal ion what is chromatography technique involves the introduction of a Sample either manually or using an autosampler, into a sample loop of known quantity. A buffered aqueous solution called the mobile phase carries the sample out of the loop into a column that comprises some kind of stationary phase material.
This is normally a resin or gel matrix which includes agarose or cellulose beads with covalently bonded charged functional groups. The target analytes anions or cations are kept on the stationary phase but may be eluted by increasing the concentration of a similarly charged species. This will displace the analyte ions in the static phase. For Instance, in cation exchange chromatography, the positively charged analyte could be displaced by the introduction of positively charged sodium ions. The analytes of interest must then be discovered by some procedure, which is normally by either conductivity or UV/Visible light absorbance. So as to restrain an ion chromatography system, a chromatography data System is usually required. Some of those chromatography data systems are also used to control gas chromatography and HPLC systems.